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US 11,630,112 B2Granted
Systems and methods for sample preparation, data generation, and protein corona analysis
AssigneeSeer, Inc.
InventorsWilliam Manning, Young Kim, Brandon Kwan-Leong, Hope Liou, Xiaoyan Zhao, Daniel Hornburg, Martin Goldberg
Life of this patent
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Abstract
Systems and methods for automated sample preparation and processing of protein corona are described herein, as well as its application in the discovery of advanced diagnostic tools as well as therapeutic agents.
Claims22
An automated system for distinguishing states of a biological sample using a plurality of magnetic particles having surfaces with different physicochemical properties, the system comprising:
- (a) a fluid transfer unit comprising a multichannel fluid transfer instrument for transferring fluids between units within the system;
- (b) a sample storage unit comprising a support for storing a plurality of biological samples;
- (c) a sensing unit comprising a support and a magnetic sensor array, wherein the magnetic sensor array is removably coupled to the support and comprises partitions that comprise the plurality of magnetic particles having surfaces with different physicochemical properties for binding a population of analytes within the biological sample, wherein the plurality of magnetic particles are configured to bind to at least 300 different proteins;
- (d) a reagent storage unit comprising supports for storing a plurality of reagents;
- (e) a waste unit comprising supports for storing a reagent to be disposed of;
- (f) a consumable storage unit comprising supports for storing consumables for use by the multichannel fluid transfer instrument;
- (g) a magnetized support, wherein the one or more processors are further programmed to transfer the magnetic sensor array to the magnetized support;
- and
- (h) a control unit comprising one or more processors programmed to perform steps comprising:
- i. contacting the biological sample with a partition of the magnetic sensor array;
- ii. incubating the biological sample with the plurality of magnetic particles of the magnetic sensor array;
- iii. removing components from the partition except the plurality of magnetic particles and the population of analytes interacting with the plurality of magnetic particles;
- iv. adding a solution to the partition such that the population of analytes are desorbed from the plurality of magnetic particles;
- and
- v. preparing the desorbed population of analytes comprising the at least 300 different proteins for mass spectrometry.
12 more elements
An automated system, the system comprising:
- (a) a fluid transfer unit comprising a multichannel fluid transfer instrument for transferring fluids between units within the system;
- (b) a sample storage unit comprising a support for storing a plurality of biological samples;
- (c) a sensing unit comprising a support and a magnetic sensor array, wherein the magnetic sensor array is removably coupled to the support and comprises partitions that comprise a plurality of nanoparticles, the plurality of nanoparticles comprising 3 to 8 types of magnetic nanoparticles having surfaces with different surface charges and/or hydrophobicity for binding a population of proteins within the biological sample, wherein the plurality of nanoparticles are configured to bind to at least 600 different proteins;
- (d) a reagent storage unit comprising supports for storing a plurality of reagents;
- (e) a waste unit comprising supports for storing a reagent to be disposed of;
- (f) a consumable storage unit comprising supports for storing consumables for use by the multichannel fluid transfer instrument;
- and
- (g) a magnetized support, wherein the one or more processors are further programmed to transfer the magnetic sensor array to the magnetized support;
- and
- (h) a control unit comprising one or more processors programmed to perform steps comprising:
- i. contacting the biological sample with a partition of the magnetic sensor array;
- ii. incubating the biological sample with the plurality of nanoparticles of the magnetic sensor array, wherein incubating the biological sample with the plurality of nanoparticles contained within the partitions of the magnetic sensor array comprises an incubation time of at least 30 minutes at a temperature between about 4° C. to about 40° C.;
- iii. removing components from the partition except the plurality of nanoparticles and the population of analytes interacting with the plurality of nanoparticles;
- iv. adding a solution to the partition such that the population of analytes are desorbed from the plurality of nanoparticles;
- and
- v. preparing the desorbed population of analytes comprising the at least 600 different proteins for mass spectrometry, wherein preparing the desorbed population of analytes for mass spectrometry comprises alkylation and digestion.
13 more elements
Dependencies are inferred from the claim text.