Methods to identify protein interaction
AssigneeAgency for Science, Technology and Research
InventorSoon Heng Tan
Life of this patent
Estimating the term…
Abstract
The present invention discloses methods for identifying a protein interaction between one or more first proteins and one or more further proteins comprising the steps of: exposing one or more samples comprising the proteins to at least one preselected condition for at least one preselected duration; isolating and separating at least one soluble fraction from an insoluble fraction of said one or more samples; and analyzing the at least one soluble fraction or the insoluble fraction to identify said protein interaction between one or more first proteins and one or more further proteins. Use of the method of the invention are also disclosed.
Claims19
A method for identifying a protein interaction between one or more first proteins and one or more further proteins, said method comprising the steps of:
- a) exposing one or more samples comprising the one or more first proteins and said one or more further proteins to at least one preselected condition for at least one preselected duration;
- b) isolating and separating at least one soluble fraction from an insoluble fraction of said one or more samples;
- c) determining and comparing the solubility of the one or more first proteins to the solubility of the one or more further proteins in the at least one soluble fraction or the insoluble fraction of step b);
- and,
- d) determining similarity in solubility between the one or more first proteins and one or more further proteins in said one or more samples, wherein a non-random increase in similarity in solubility is indicative of a protein interaction between said one or more first proteins and said one or more further proteins in said one or more samples.
Dependencies are inferred from the claim text.