Methods of using self-heating biosensor based on lossy mode resonance

A method of using a self-heating biosensor based on lossy mode resonance, comprising the steps of:

• placing an object to be tested on a biological material sensing region with bioprobes of a lossy mode resonance layer,
• inputting an incident light from a broadband light source to a waveguide unit disposed under the lossy mode resonance layer,
• measuring a light outputted from the waveguide unit by a spectrometer, and
• heating the biomaterial sensing region by energizing the lossy mode resonance layer,
• wherein the bioprobes are consisting of a boride functional group, a formation of the boride functional group comprises:
• sequentially cleaning the lossy mode resonance layer with acetone, absolute ethanol, ultrapure water, potassium hydroxide aqueous solution, and ultrapure water;
• cleaning the lossy mode resonance layer with a mixed solution of ammonia water and hydrogen peroxide to remove organic contaminants and generate hydroxyl groups (OH) by a hydroxylation treatment;
• attaching the hydroxyl groups to a silane and leaving an end with isocyanate to be combined with boric acid by a salinization treatment;
• removing a carboxyl group (COON) to bond an isocyanate group to a benzene ring by a decarboxylation treatment;
• wherein carbon dioxide is generated by the lossy mode resonance layer during a reaction, bubbles generation is observed, and the boride functional group is formed.