Method for diagnosing anomalies in the coagulation of blood

A method for determining the efficacy of a coagulant or anticoagulant treatment of a blood clotting anomaly in a subject, comprising the following steps:

• i) determining the value for the area under the curve of impedance of a blood sample from said subject before the beginning of said treatment by carrying out the steps of:
• a) placing the blood sample, from said subject before the beginning of said treatment, in a receptacle containing two pairs of electrodes connected to a device generating an electric current;
• b) incubating this sample for 60 to 180 seconds in the presence of calcium;
• c) adding to this sample a tissue factor in a final concentration of 0.5 to 5 pM;
• d) measuring the impedance variation between the electrodes as a function of time for a period of 10 to 30 minutes from step (c) and generating a curve of the impedance values as a function of time,
• ii) treating the subject with said treatment, and
• iii) determining the value for the area under the curve of impedance of a blood sample from said subject after the beginning of step ii) by carrying out the steps of:
• a) placing the blood sample, from said subject after the beginning of said treatment, in a receptacle containing two pairs of electrodes connected to a device generating an electric current;
• b) incubating this sample for 60 to 180 seconds in the presence of calcium;
• c) adding to this the sample tissue factor in a final concentration of 0.5 to 5 pM;
• d) measuring the impedance variation between the electrodes as a function of time for a period of 10 to 30 minutes from step (c) and generating a curve of the impedance values as a function of time,
• with said treatment being considered effective when the value for the area under the curve of impedance determined in step iii) is greater than the value for the area under the curve of impedance determined in step i).