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US 11,630,092 B2Granted
Online chromatography and electrospray ionization mass spectrometer
AssigneeRegeneron Pharmaceuticals, Inc.
InventorQian Zhang
Life of this patent
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Abstract
Methods and system for protein characterization using online chromatography and electrospray ionization mass spectrometry are provided.
Claims18
A method for characterizing an antibody-drug conjugate, said method comprising:
- contacting a sample including the antibody-drug conjugate to a chromatographic system having a size-exclusion chromatography resin;
- washing said size-exclusion chromatography resin using a mobile phase to provide an eluent including the antibody-drug conjugate;
- and
- characterizing the antibody-drug conjugate in said eluent using an electrospray ionization mass spectrometer under native conditions.
1 more element
A system comprising:
- a chromatographic column having a size-exclusion chromatography resin, wherein the chromatographic column is capable of receiving a mobile phase and a sample including a protein, wherein the chromatographic column is coupled to an ultraviolet detector using a T-splitter, and
- an electrospray ionization mass spectrometer, wherein the electrospray ionization mass spectrometer is capable of being coupled to said chromatographic column and wherein the electrospray ionization mass spectrometer is capable of being run under native conditions.
Dependencies are inferred from the claim text.