Back to results
US 11,630,066 B2Granted
Large-field 3D spectral microscopy
AssigneesAlveole, Centre National de la Recherche Scientifique (CNRS), Universite De Bordeaux
InventorsVincent Studer, Rémi Galland
Life of this patent
Estimating the term…
Abstract
Optical device for optical fluorescence microscopy, comprising a spatial modulator (1), a microscope objective (2), a beamsplitter (3) and a camera (4), wherein the beamsplitter (3) is placed between the spatial modulator (1) and the microscope objective (2), wherein the beamsplitter (3) is placed between the camera (4) and the microscope objective (2), and wherein a prism (5) is placed between the beamsplitter (3) and the camera (4).
Claims9
A method for calibrating, at a first wavelength, a wide-field microscope, comprising the following steps:
- placing in a vicinity of an object plane an object medium able to emit fluorescence light in a first spectral band containing the first wavelength, in response to first illumination light,
- wherein the wide-field microscope comprises:
- a dichroic beamsplitter;
- a spatial modulator;
- a camera;
- a first optical channel that uses the spatial modulator to transmit the first illumination light;
- a second optical channel extending between the dichroic beamsplitter and the object plane in a three dimensional object medium able to emit fluorescence light in response to the first illumination light, wherein the second optical channel transmits the first illumination light and the fluorescence light, and wherein the dichroic beamsplitter is dichroic with respect to the first illumination light and fluorescence light;
- a third optical channel extending between the dichroic beamsplitter and the camera, wherein the third optical channel transmits the fluorescence light from the dichroic beamsplitter to the camera that detects the fluorescence light,
- wherein the spatial modulator limits a field of the wide-field microscope,
- wherein the wide-field microscope further comprises an optical means for conjugating the spatial modulator with the object plane for the first illumination light and for conjugating the object plane with the camera for the fluorescence light, and
- wherein the third optical channel comprises an optical element that is dispersive to the fluorescence light,
- placing in the third optical channel a first interference filter able to spectrally select a vicinity of the first wavelength,
- sequentially illuminating, with the first illumination light, each point of the wide field of the wide-field microscope in the object plane, by means of the spatial modulator,
- recording a first image of each point of the wide field of the wide-field microscope, with the camera,
- removing the first interference filter from the third optical channel.
12 more elements
Dependencies are inferred from the claim text.