US 11,630,059 B2Granted

Interference image acquiring device and method for acquiring interference image

AssigneesHamamatsu Photonics K.K., National University Corporation Hamamatsu University School of Medicine

InventorsToyohiko Yamauchi, Kentaro Goto, Hisayuki Matsui, Satoshi Hirakawa, Hidenao Yamada

Life of this patent

Estimating the term…

Loading patent…

Abstract

An interference image acquisition apparatus includes a light source, a beam splitter, a second reflection mirror, an imager, and a first reflection mirror. A cell is placed on one side of a transparent material, and the first reflection mirror is placed on the other side of the transparent material. In a two-beam interferometer, an optical path difference between an optical path length of a first light beam reflected by the first reflection mirror and an optical path length of a second light beam reflected by the second reflection mirror is set to a coherence length of light output from the light source or less. The imager acquires an interference image in a state in which the cell is placed at a position conjugate to an imaging plane in a first optical system between the imaging plane and the first reflection mirror.

Claims22

Dependent claims

An interference image acquisition apparatus comprising:

a light source configured to output incoherent light;
a two-beam interferometer configured to split the light output from the light source into a first light beam and a second light beam, combine the first light beam transmitted through an object placed on an optical path of the first light beam and reflected by a first reflection mirror and the second light beam reflected by a second reflection mirror, and output interference light;
and
an imager having an imaging plane configured to receive the interference light output from the two-beam interferometer, the imager configured to acquire an interference image of the object, wherein
in the two-beam interferometer, the first reflection mirror is placed apart from the object, and an optical path difference between an optical path length of the first light beam reflected by the first reflection mirror and an optical path length of the second light beam reflected by the second reflection mirror is set to a coherence length of the light output from the light source or less, and
the imager is configured to acquire the interference image in a state in which the object is placed at a position conjugate to the imaging plane in a first optical system between the imaging plane and the first reflection mirror.

3 more elements

An interference image acquisition method comprising:

an interference step of, in a two-beam interferometer, splitting incoherent light output from a light source into a first light beam and a second light beam, combining the first light beam transmitted through an object placed on an optical path of the first light beam and reflected by a first reflection mirror and the second light beam reflected by a second reflection mirror, and outputting interference light;
and
an imaging step of acquiring an interference image of the object by an imager having an imaging plane configured to receive the interference light output from the two-beam interferometer, wherein
in the interference step, in the two-beam interferometer, the first reflection mirror is placed apart from the object, and an optical path difference between an optical path length of the first light beam reflected by the first reflection mirror and an optical path length of the second light beam reflected by the second reflection mirror is set to a coherence length of the light output from the light source or less, and
in the imaging step, the interference image is acquired by the imager in a state in which the object is placed at a position conjugate to the imaging plane in a first optical system between the imaging plane and the first reflection mirror.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application: 16/962,024Filed 22 Nov 2018
Priority: JP JP2018-00468116 Jan 2018
Published: US 2020/0400562 A124 Dec 2020
Granted: 18 Apr 2023
Term ends: Not estimatedThe source records don’t support an estimate.

Classification23 codes

C12N 5/0668
Stem cells: mesenchymal stem cells from other natural sources
G01N 21/45
Refractivity; Phase-affecting properties, e.g. optical path length: using interferometric methods; using Schlieren methods
C12M 41/46
Means for regulation, monitoring, measurement or control, e.g. flow regulation: of cellular or enzymatic activity or functionality, e.g. cell…
C12N 5/0062
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor: general…