Parallel flow cytometer using radiofrequency multiplexing
AssigneeThe Regents of the University of California
InventorsBahram Jalali, Eric D. Diebold, Brandon Buckley
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Abstract
An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.
Claims20
An apparatus comprising:
- a light beam generator component configured to generate a local oscillator beam and plurality of radiofrequency-shifted beams of light;
- an optical component configured to combine the local oscillator beam and the plurality of radiofrequency-shifted beams of light and direct the combined beam onto a flow cell;
- and
- a photodetector.
A method comprising:
- irradiating a sample comprising particles in a flow cell with a local oscillator beam and plurality of radiofrequency-shifted beams of light;
- and
- detecting light from the irradiated particles in the sample with a photodetector.
Dependencies are inferred from the claim text.