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US 11,629,434 B2Granted
Antibody screening methods
AssigneeX-Body, Inc.
InventorsYan Chen, Tod M. Woolf, Richard W. Wagner
Life of this patent
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Abstract
Provided are methods and compositions for the production of novel antibodies that bind specifically to a target antigen. These methods and compositions are particularly useful for producing antibodies having the antigen binding specificity of a reference antibody but with improved properties (e.g., binding affinity, immunogenicity, and thermodynamic stability) relative to the reference antibody.
Claims10
A method for selecting a bispecific, stable VH/VL pair, the method comprising:
- (a) providing an unpaired VH domain that binds specifically to a first antigen;
- (b) contacting the unpaired VH domain with a library of unpaired VL domains such that a library of VH/VL pairs is formed;
- (c) contacting the library of VH/VL pairs with a second antigen;
- (d) selecting from the library of VH/VL pairs at least one VH/VL pair that binds specifically to the second antigen;
- (e) contacting the VH/VL pair(s) selected in step (d) with the first antigen;
- and
- (f) selecting at least one VH/VL pair that binds specifically to the first antigen, thereby selecting a bispecific, stable VH/VL pair.
4 more elements
Dependencies are inferred from the claim text.