Delaying real-time sequencing

A composition comprising a plurality of nucleic acid sequencing templates, wherein each of the plurality of nucleic acid sequencing templates comprise:

• an adaptor comprising a priming region and a runway region, wherein the adaptor is connected to both strands of a double-stranded insert region at one end of the double-stranded insert region, wherein the priming region of the adaptor is disposed either within a single stranded portion of a hairpin\or within a single stranded overhang, the priming region further comprising a primer complementary to at least a portion of the priming region, and a strand displacing polymerase bound to the template at the priming region to form a polymerase-template complex, wherein the double-stranded insert region comprises a sequence of interest, wherein the runway region is between the priming region and the double-stranded insert region, whereby, when the polymerase extends the primer, it first extends through the runway region and then extends through the insert region, wherein the runway region comprises a secondary structure with a hairpin, and wherein the runway region comprises DNA.