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US 11,629,375 B2Granted
Methods and kits for determining the efficiency of plasma separation from whole blood
AssigneeNucleix Ltd.
InventorsDanny Frumkin, Adam Wasserstrom, Revital Knirsh
Life of this patent
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Abstract
Methods and kits for determining the efficiency of plasma separation from whole blood are provided, using real-time PCR amplification of two amplicons, namely, a short amplicon of e.g. 70-150 bps and a long amplicon of e.g. 350-600 bps. The separation efficiency is determined based on the difference in amplification patterns of the two amplicons.
Claims14
A method for determining the efficiency of plasma separation from whole blood, the method comprising:
- (a) obtaining DNA from a plasma sample;
- (b) generating by PCR co-amplification:
- (i) a first amplification product of 70-150 bps from a first genomic locus using a first primer pair, wherein the first amplification product comprises the sequence set forth in SEQ ID NO:
- 1 or SEQ ID NO:
- 10, and
- (ii) a second amplification product of at least 350 bps from a second genomic locus using a second primer pair, wherein the second amplification product comprises the sequence set forth in one of SEQ ID NO:
- 2, SEQ ID NO:
- 7, SEQ ID NO:
- 8, or SEQ ID NO:
- 9;
- (c) calculating a signal intensity for each of said first and second amplification products;
- and
- (d) determining that the plasma sample is separated when the difference between the signal intensities is above a predefined threshold;
- wherein said first and second amplification products produce distinct signal intensity differences for plasma DNA and whole blood DNA.
11 more elements
Dependencies are inferred from the claim text.