Article and methods to determine efficacy of disinfection process

A process challenge device, comprising:

• a body with a hollow channel having a first aperture and a second aperture spaced apart from the first aperture;
• and
• an article fixedly disposed in the hollow channel, the article comprising:
• a nonwoven substrate having a copolymer grafted thereto, the copolymer comprising interpolymerized monomer units of
• a cationic nitrogen-containing ligand monomer selected from quaternary ammonium-containing and/or guanidinyl-containing ligand monomers;
• an amide monomer;
• an oxy monomer;
• wherein the grafted copolymer comprises:
• a. 10 to 50 parts by weight of the cationic nitrogen-containing ligand monomer;
• b. 10 to 80 parts by weight of the amide monomer;
• c. 10 to 40 parts by weight of the oxy monomer;
• and
• d. 0 to 30 parts by weight of a poly(alkylene oxide) monomer,
• wherein the sum of a) to d) is 100 parts by weight;
• and
• a dried coating adhered to the substrate, the coating comprising
• an optional water-soluble or water-dispersible polymeric binding agent;
• and
• a plurality of test microorganisms.