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US 11,629,369 B2Granted
Electrochemical strips for monitoring the degradation of body fat and method for preparing same
AssigneeLsee
InventorsSébastien Delile, Cyril Torre
Life of this patent
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Abstract
Devices, systems, and methods are used for personalized monitoring of changes in metabolism as a function of external parameters such as food or physical exercise. More particularly, the present disclosure relates to electrochemical strips for detecting the amount of biomarker for fat metabolism, in particular, glycerol.
Claims12
An electrochemical test strip for detecting glycerol in a body fluid sample, comprising:
- an electrically insulating substrate layer;
- at least two electrodes, at least a first electrode of the at least two electrodes comprising a working electrode comprising carbon and being functionalized with ferrocene derivatives in a reduced form, and at least a second electrode of the at least two electrodes comprising a reference electrode and a counter electrode;
- at least as many electrical tracks as the number of the at least two electrodes;
- an enzyme layer disposed on the at least two electrodes, the enzyme layer comprising glycerol-specific enzymes, the glycerol-specific enzymes selected from among the group consisting of:
- glycerol kinase and glycerol phosphate oxidase;
- a layer of co-factors selected from among the group consisting of:
- adenosine triphosphate and MgCl2;
- and
- at least one membrane layer;
- a top layer;
- and
- a spacer layer located between the at least two electrodes and the top layer, the spacer layer creating a space defining a measuring chamber for receiving the sample, the measuring chamber at least partially covering the electrodes and the enzyme layer.
9 more elements
A method of forming an electrochemical strip, comprising:
- providing an electrically insulating substrate layer;
- forming at least two electrodes, at least a first electrode of the at least two electrodes comprising a working electrode comprising carbon and being functionalized with ferrocene derivatives in a reduced form, and at least a second electrode of the at least two electrodes comprising a reference electrode and a counter electrode;
- forming at least as many electrical tracks as the number of the at least two electrodes;
- forming an enzyme layer disposed on the at least two electrodes and containing glycerol-specific enzymes consisting of:
- glycerol kinase and glycerol phosphate oxidase;
- and
- forming a layer of co-factors consisting of:
- adenosine triphosphate and MgCl2;
- and
- forming at least one membrane layer;
- forming a spacer layer;
- and
- forming a top layer, the spacer layer disposed between the at least two electrodes and the top layer.
10 more elements
Dependencies are inferred from the claim text.