US 11,629,363 B2Granted

Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 1,2-propanediol, n-propanol, 1,3-propanediol, or glycerol related thereto

AssigneeGenomatica, Inc.

InventorsAnthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae

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Abstract

Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.

Claims20

Dependent claims

A non-naturally occurring microbial organism comprising:

(A) a methanol metabolic pathway, wherein said organism comprises at least one exogenous nucleic acid encoding a methanol metabolic pathway enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of methanol, wherein said methanol metabolic pathway comprises:
(i) a methanol methyltransferase and a methylenetetrahydrofolate reductase;
(ii) a methanol dehydrogenase;
or
(iii) a methanol dehydrogenase and a formaldehyde activating enzyme;
and
(B) (1) a 1,3-propanediol pathway comprising (1) (i) a glyceraldehyde-3-phosphate reductase;
(ii) a glycerol-3-phosphate phosphatase or a glycerol kinase;
(iii) a glycerol dehydratase;
and (iv) a 3-hydroxypropanal reductase;
(2)(i) a dihydroxyacetone phosphate reductase;
(ii) a glycerol-3-phosphate phosphatase or a glycerol kinase;
(iii) a glycerol dehydratase;
and (iv) a 3-hydroxypropanal reductase;
or (3)(i) a dihydroxyacetone phosphate phosphatase or a dihydroxyacetone kinase;
(ii) a dihydroxyacetone reductase;
(iii) a glycerol dehydratase;
and (iv) a 3-hydroxypropanal reductase;
or
(2) a glycerol pathway comprising (1)(i) a glyceraldehyde-3-phosphate reductase;
and (ii) a glycerol-3-phosphate phosphatase or a glycerol kinase;
(2)(i) a dihydroxyacetone phosphate reductase;
and (ii) a glycerol-3-phosphate phosphatase or a glycerol kinase;
or (3)(i) a dihydroxyacetone phosphate phosphatase or a dihydroxyacetone kinase;
and (ii) a dihydroxyacetone reductase.

22 more elements

Dependencies are inferred from the claim text.

Facts

Application: 15/873,798Filed 17 Jan 2018 · continuation
Priority: 5 Nov 2012
Published: US 2018/0327788 A115 Nov 2018
Granted: 18 Apr 2023
Term ends: Not estimatedThe source records don’t support an estimate.

Classification18 codes

C12P 7/24
Preparation of oxygen-containing organic compounds: containing a carbonyl group
C12N 1/14
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…
C12N 1/16
Fungi ; Culture media therefor: yeasts; Culture media therefor
C12N 1/20
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…

Family

Application61/722,629Us provisional application · filed 5 Nov 2012
Application61/766,635Us provisional application · filed 19 Feb 2013
Application14/071,318Parent of this continuation · filed 4 Nov 2013
US 2018/0327788 A1Pre-grant publication · published 15 Nov 2018
US 9,909,150 B2Parent of this continuation
US 2014/0302575 A1Published application
US 11,629,363 B2This patent

From the US filing history. Foreign family members aren’t listed.