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US 11,629,357 B2Granted
DNA constructs for obtaining marker-free transgenic plants
AssigneeMonsanto Technology LLC
InventorsXudong Ye, Larry A. Gilbertson, Shihshieh Huang, Susan J. Johnson, Michael W. Petersen, David Walters, Paul S. Chomet
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Abstract
The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
Claims20
A DNA construct comprising
- (a) a first DNA segment comprising a gene of interest;
- and
- (b) a second DNA segment comprising a promoter functional in a seed operably linked to a DNA cassette that confers a detectable phenotype in seeds comprising the DNA cassette and a marker gene operably linked to a promoter functional in plants;
- further wherein the detectable phenotype comprises a change of:
- seed size, seed opacity, seed germinability, seed viability, seed shape, or seed texture.
2 more elements
A transgenic plant co-transformed with a first DNA construct and a second DNA construct,
- wherein the first DNA construct comprises a first DNA segment comprising left and right T-DNA borders flanking a gene of interest operably linked to a promoter functional in plants, and
- wherein the second DNA construct comprises a second DNA segment comprising a second set of left and right T-DNA borders flanking a promoter functional in a seed operably linked to a DNA cassette that confers a detectable phenotype in seeds comprising the DNA cassette and a marker gene operably linked to a promoter functional in plants, wherein the detectable phenotype comprises a change of:
- seed size, seed opacity, seed germinability, seed viability, seed shape, or seed texture.
Dependencies are inferred from the claim text.