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US 11,629,351 B2Granted
Method for suppressing methylation of target DNA in plant
AssigneeNational Institute of Advanced Industrial Science and Technology
InventorsChikara Masuta, Tsuyoshi Inukai, Wataru Matsunaga, Reika Isoda, Takeshi Matsumura, Go Atsumi
Life of this patent
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Abstract
Plants are created with desired traits by conveniently and rapidly inhibiting methylation of target DNA in plants, without using recombination technology. Scaffold RNA produced by transcription of target DNA is cleaved in an RNA-directed DNA methylation mechanism.
Claims6
A method for inhibiting methylation of a 35S promoter in a plant cell, wherein the method comprises cleaving a scaffold RNA produced by transcription of the 35S promoter in an RNA-directed DNA methylation mechanism in the plant cell;
- wherein the scaffold RNA is cleaved by expressing one or more ribozymes specific for the scaffold RNA,
- the one or more ribozymes is introduced into the plant cell by a plant virus vector method or an agroinfiltration method, and
- the plant is a Poaceae, Leguminosae, Brassicaceae, Compositae, Solanaceae, Rosaceae, Cucurbitaceae, or Convolvulaceae plant.
Dependencies are inferred from the claim text.