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US 11,629,344 B2Granted
Methods and systems for processing polynucleotides
Assignee10x Genomics, Inc.
InventorsBenjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov
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Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
Claims54
A method for sample preparation, the method comprising:
- (a) contacting a plurality of cells with a plurality of beads comprising a plurality of oligonucleotides comprising barcode sequences to form a plurality of constructs, each construct of the plurality of constructs comprising a cell of the plurality of cells and a bead of the plurality of beads, wherein the cell comprises polynucleotides,
- (b) encapsulating the plurality of constructs together in a matrix, and
- (c) generating a plurality of nucleic acid molecules comprising the barcode sequences of oligonucleotides of the plurality of oligonucleotides, each nucleic acid molecule of the plurality of nucleic acid molecules comprising a barcode sequence of the barcode sequences or complement thereof and at least a portion of a polynucleotide of the polynucleotides, or complement thereof, of the cell.
A method for sample preparation, the method comprising:
- (a) contacting a plurality of eukaryotic organelles with a plurality of beads comprising a plurality of oligonucleotides comprising barcode sequences to form a plurality of constructs, each construct of the plurality of constructs comprising a eukaryotic organelle of the plurality of eukaryotic organelles and a bead of the plurality of beads, wherein the eukaryotic organelle comprises polynucleotides,
- (b) encapsulating the plurality of constructs together in a matrix,
- (c) generating a plurality of nucleic acid molecules comprising the barcode sequences of the oligonucleotides, each nucleic acid molecule of the plurality of nucleic acid molecules comprising a barcode sequence of the barcode sequences or complement thereof and at least a portion of a polynucleotide of the polynucleotides, or complement thereof, of the eukaryotic organelle.
Dependencies are inferred from the claim text.