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US 11,629,340 B2Granted
DHFR tunable protein regulation
AssigneeObsidian Therapeutics, Inc.
InventorsVipin Suri, Dan Jun Li, Dexue Sun, Byron Delabarre, Vijaya Balakrishnan, Brian Dolinski, Mara Christine Inniss, Grace Y. Olinger
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Abstract
The present invention is related to compositions and methods for the regulated and controlled expression of proteins.
Claims29
A biocircuit system comprising at least one effector module, said effector module comprising
- (a) a stimulus response element (SRE) and
- (b) at least one payload, said payload comprising a protein of interest which is fused to said SRE, wherein said SRE comprises a destabilizing domain (DD), said DD comprising a human dihydrofolate reductase (hDHFR) mutant selected from the group consisting of a hDHFR comprising the amino acid sequence of SEQ ID NO. 6445;
- a hDHFR comprising the amino acid sequence of SEQ ID NO. 6447;
- a hDHFR comprising the amino acid sequence of SEQ ID NO. 6451;
- a hDHFR comprising the amino acid sequence of SEQ ID NO. 6452;
- a hDHFR comprising the amino acid sequence of SEQ ID NO. 6453;
- a hDHFR comprising the amino acid sequence of SEQ ID NO. 6454;
- and a hDHFR comprising the amino acid sequence of SEQ ID NO. 6469.
5 more elements
An effector module comprising a hDHFR-derived SRE fused to a payload, wherein the hDHFR-derived SRE is a hDHFR mutant selected from the group consisting of a hDHFR comprising the amino acid sequence of SEQ ID NO. 6445;
- a hDHFR comprising the amino acid sequence of SEQ ID NO. 6447;
- a hDHFR comprising the amino acid sequence of SEQ ID NO. 6451;
- a hDHFR comprising the amino acid sequence of SEQ ID NO. 6452;
- a hDHFR comprising the amino acid sequence of SEQ ID NO. 6453;
- a hDHFR comprising the amino acid sequence of SEQ ID NO. 6454;
- and a hDHFR comprising the amino acid sequence of SEQ ID NO. 6469.
3 more elements
Dependencies are inferred from the claim text.