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US 11,629,339 B2Granted
Aseptic purification process for viruses
AssigneeValneva SE
InventorsRobert Schlegl, Michael Weber
Life of this patent
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Abstract
Described herein are processes for the purification of viruses and compositions thereof.
Claims12
A process of purification of virus particles wherein the virus particles belong to the Coronaviridae virus family, comprising the steps of:
- (a) providing a liquid medium comprising said virus particles, wherein the virus particles are greater than or equal to about 100 nm in diameter;
- (b) contacting the virus particles with a solid-phase matrix comprising a ligand-activated core and an inactive shell comprising pores having a molecular weight cut off that excludes the virus particles from entering the ligand-activated core, and wherein a molecule smaller than the molecular weight cut off of the pores can enter the ligand-activated core;
- and
- (c) separating the solid-phase matrix from the virus particles to produce a final virus preparation;
- wherein the solid-phase matrix is added to the liquid medium comprising the virus particles at a final concentration between 2.5% (v/v) and 30% (v/v);
- and wherein each step of the process is performed aseptically.
3 more elements
A method for manufacturing a composition for immunization against a viral infection caused by a virus of the Coronaviridae family comprising the steps of:
- (a) providing a liquid medium comprising virus particles belonging to the Coronaviridae family, wherein the virus particles are greater than or equal to about 100 nm in diameter;
- (b) contacting the virus particles with a solid-phase matrix comprising a ligand-activated core and an inactive shell comprising pores having a molecular weight cut off that excludes the virus particles from entering the ligand-activated core, and wherein a molecule smaller than the molecular weight cut off of the pores can enter the ligand-activated core;
- (c) separating the solid-phase matrix from the virus particles to produce a final virus preparation;
- and
- (d) obtaining a sterile composition comprising the highly purified virus preparation,
- wherein the solid-phase matrix is added to the liquid medium comprising the virus particles at a final concentration between 2.5% (v/v) and 30% (v/v);
- and wherein each step of the process is performed aseptically.
4 more elements
Dependencies are inferred from the claim text.