Method for acclimating and suspending Vero and second order production process for virus

A second-order cultivation method for producing vaccines with suspension Vero cells, comprising the following steps:

• 1) cell growth:
• suspension Vero cells are grown in a growth medium to 2.0×106 cells/mL˜20.0×106 cells/mL;
• 2) inoculation:
• the inoculation amount is between 10−1˜10−5 MOIs, or viruses are inoculated after step 3) according to this inoculation amount;
• 3) dilution/supplementation of a production medium:
• Diluting 1 to 5 times with the production medium, and after dilution, the cell density reaches 1.0×106 cells/mL to 10.0×106 cells/mL;
• 4) secondary growth:
• secondary growth of cells;
• 6) optionally, the virus solution is harvested and purified for vaccine preparation, wherein the suspension Vero cells are suspension cells used for vaccine production, or suspension Vero cells acclimated from adherent Vero cells and capable of suspension growth and production which are obtained from adherent Vero cells by low serum acclimation or serum-free acclimation and by suspension acclimation, wherein “serum-free acclimation” means that the acclimated Vero cells can grow and proliferate in a medium without serum.