Gamma delta T cells and a method of augmenting the tumoricidal activity of the same
AssigneeAgency for Science, Technology and Research
InventorsShu Wang, Wei Xia Ang, Jieming Zeng
Life of this patent
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Abstract
The present invention relates to a method of generating γδ T cells having at least one down-regulated co-inhibitory receptor, the method comprising the steps of: (a) culturing a population of cells comprising γδ T cells with a phosphoantigen to expand the γδ T cells; and (b) culturing the expanded γδ T cells with artificial antigen-presenting cells expressing a Fc receptor, and an anti-CD3 antibody. The present invention also relates to γδ T cells generated according to a method of the present invention, as well as methods of treatment and medical uses thereof.
Claims14
A method of generating γδ T cells having at least one down-regulated co-inhibitory receptor, the method comprising the steps of:
- (a) culturing a population of cells comprising Vγ9Vδ2 T cells with a phosphoantigen to expand the Vγ9Vδ2 T cells;
- (b) culturing the expanded Vy9V62T cells with artificial antigen-presenting cells expressing a Fc receptor, and an anti-CD3 antibody;
- and
- (c) modifying the Vγ9Vδ2 T cells to express a chimeric antigen receptor (CAR) wherein the CAR overexpresses an extracellular antigen binding domain of natural killer group 2D (NKG2D).
Dependencies are inferred from the claim text.