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US 11,629,319 B2Granted
Cell culture device and methods
AssigneeMimetas B.V.
InventorsPaul Vulto, Sebastiaan Johannes Trietsch, Arnaud Nicolas
Life of this patent
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Abstract
A method of vascularising a cell aggregate on a microfluidic device, microfluidic cell culture devices comprising perfusable vascular networks and kits and assays using the microfluidic cell culture devices are described. The microfluidic devices comprise one or more capillary pressure barriers allowing for formation of an extracellular matrix gel within a confined area of the network, in which cells can be cultured for different uses.
Claims20
A cell culture device comprising a microfluidic network, the microfluidic network comprising:
- a microfluidic layer comprising a base, a microfluidic channel and a cover;
- an organoid compartment extending into the microfluidic layer through an aperture in the cover and in fluid communication with the microfluidic channel;
- and
- a first capillary pressure barrier substantially aligned with the aperture and dividing the microfluidic network into a first sub-volume comprising the organoid compartment and a second sub-volume comprising at least a part of the microfluidic channel.
1 more element
Dependencies are inferred from the claim text.