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US 11,629,318 B2Granted
Methods for producing mature adipocytes and methods of use thereof
AssigneePresident and Fellows of Harvard College
InventorsBenjamin D. Pope, Kevin Kit Parker
Life of this patent
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Abstract
The present invention provides methods and systems which accommodate 3-dimensional adipocyte expansion to produce, e.g., mature adipocytes and synthetic adipose tissue with cellular properties of mature adult organisms, including cell size and cytoarchitecture, and the use of such methods and systems for, e.g., in vitro drug screening and/or toxicity assays, disease modeling, and therapeutic applications.
Claims17
A method for producing one or more mature adipocytes in vitro, comprising;
- seeding a plurality of mesenchymal progenitor cells into a seeding site;
- culturing the mesenchymal progenitor cells under conditions that induce adipogenesis of one or more of the mesenchymal progenitor cells, thereby producing one or more adipocytes;
- disposing a fiber layer over the one or more adipocytes, the fiber layer comprising a plurality of polymer fibers each having a diameter in a range of about 100 nm to about 1 μm;
- and
- culturing the one or more adipocytes with the fiber layer over the one or more adipocytes under suitable conditions until each of the one or more adipocytes grows to a diameter of between about 30 μm and about 300 μm, thereby generating one or more mature adipocytes in vitro.
2 more elements
A method for producing a tissue-engineered food product, the method comprising:
- seeding a plurality of mesenchymal progenitor cells into a seeding site;
- culturing the mesenchymal progenitor cells under conditions that induce adipogenesis of one or more of the mesenchymal progenitor cells, thereby producing one or more adipocytes;
- disposing a fiber layer over the one or more adipocytes, the fiber layer comprising a plurality of polymer fibers each having a diameter in a range of about 100 nm to about 1 μm;
- culturing the one or more adipocytes with the fiber layer over the one or more adipocytes under suitable conditions until each of the one or more adipocytes grows to a diameter of between about 30 μm and about 300 μm, thereby generating one or more mature adipocytes;
- and
- combining the one or more mature adipocytes with an engineered muscle tissue product to produce an engineered meat, thereby producing a tissue-engineered food product.
3 more elements
Dependencies are inferred from the claim text.