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US 11,629,309 B2Granted
Solvent free process for extraction of cholesterol from milk fat
AssigneeFermenta Biotech Limited
InventorsAnupama Datla, Prashant Nagre, Jagdish Tamore, Sreenath Trivikram, Amol Shirsath
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Abstract
The present invention discloses solvent free process for extracting cholesterol free of impurities from milk fat. The so isolated cholesterol is useful for the further preparation of vitamin D3. The present invention further provides pharmaceutical grade cholesterol from milk fat of high purity.
Claims13
A process for extraction of cholesterol from milk fat, comprising:
- i. a solvent-free step of heating un-saponified milk fat with anhydrous calcium chloride to obtain a CaCl2-cholesterol adduct;
- and
- ii. separating cholesterol from the CaCl2-cholesterol adduct by refluxing the CaCl2-cholesterol adduct in an organic solvent, followed by re-crystallization of cholesterol.
A process for synthesis of vitamin D3, comprising:
- extracting cholesterol from milk fat by:
- heating un-saponified milk fat with anhydrous calcium chloride in a molar ratio ranging from 1:1 to 1:12 to obtain a CaCl2-cholesterol adduct;
- and
- separating cholesterol from the CaCl2-cholesterol adduct by refluxing the CaCl2-cholesterol adduct in a solvent;
- converting the separated cholesterol to 7-dehydrocholesterol (7-DHC);
- and
- converting 7-DHC to vitamin D3 by irradiation.
4 more elements
Dependencies are inferred from the claim text.