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US 11,629,178 B2Granted
Dual cytokine fusion proteins comprising IL-10
AssigneeDeka Biosciences, Inc.
InventorJohn Mumm
Life of this patent
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Abstract
The application relates to a dual cytokine fusion protein composition, pharmaceutical composition, and/or formulation thereof comprising IL-10 or IL-10 variant molecules fused to a single chain variable fragment scaffolding system and a second cytokine, where the second cytokine is linked in the hinge region of the scFv. The application also relates to methods of using the dual cytokine fusion protein composition for treating cancer, inflammatory diseases or disorders, and immune and immune mediated diseases or disorders.
Claims6
A dual cytokine fusion protein of formula (I) NH2-(IL-10)-(X1)—(Zn)—(X2)—(IL-10)-COON (Formula I);
- wherein
- “IL-10” is a monomer;
- “X1” is a VL or VH region from a first monoclonal antibody;
- “X2” is a VH or VL region from the first monoclonal antibody;
- wherein when X1 is a VL, X2 is a VH or when X1 is a VH, X2 is a VL,
- wherein the first monoclonal antibody is an anti Ebola antibody;
- wherein the VL and VH from the anti-Ebola antibody include 3 light chain CDRs and 3 heavy chain CDRs that are engrafted with 3 light chain CDRs and 3 heavy chain CDRs from a second monoclonal antibody;
- “Z” is a cytokine other than IL-10;
- “n” is an integer of 1;
- and
- wherein:
- the IL-10 is SEQ ID No:
- 14, the second antibody is an anti-CD14 monoclonal antibody, and Z is IL-4.
10 more elements
Dependencies are inferred from the claim text.