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US 11,629,175 B2Granted
Method for preparing PHFS-like Tau aggregates
AssigneeEcole Polytechnique Federale De Lausanne (Epfl)
InventorsHilal A. Lashuel, Nadine Ait-Bouziad
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Abstract
The invention relates to a method for preparing PHFs-like Tau aggregates and to a method for identifying compounds that are inhibitors of Tau protein aggregation, blockers of Tau seeding and propagation, and imaging agents that specifically bind PHF.
Claims6
A method for preparing paired-helical filaments (PHFs)-like Tau aggregates comprising:
- (a) contacting R2 fragments consisting of SEQ ID NO:
- 1 with polyanions,
- (b) allowing formation of R2 fibrils,
- (c) breaking down the R2 fibrils into seeds,
- (d) contacting Tau proteins comprising SEQ ID NO:
- 1 with the R2 fibril seeds under conditions which allow Tau aggregation, wherein the Tau proteins comprising SEQ ID NO:
- 1 are selected from the group consisting of full length Tau proteins 4R2N (SEQ ID NO:
- 5), Tau microtubule binding domain (K18) (SEQ ID NO:
- 6), Tau protein 4R1N (SEQ ID NO:
- 7), Tau protein 4R0N (SEQ ID NO:
- 8), mouse Tau isoform m4R2N (SEQ ID NO:
- 9), mouse Tau isoform m4R0N (SEQ ID NO:
- 10) and a combination thereof.
10 more elements
Dependencies are inferred from the claim text.