Method of synthesizing 18F radiolabeled biomolecular agents
AssigneeArizona Board of Regents on Behalf of the University of Arizona
InventorsMark D. Pagel, Iman Daryaei, Abigail Shepard
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Abstract
A method for preparing 18F radiolabeled biomolecules and agents for 18F-PET imaging is disclosed herein. A perfluoroaryl-conjugated target tracer is synthesized and purified with temperature and solvent conditions that are mild for the tracer molecule. The purified perfluoroaryl-conjugated target tracer is then labeled with 18F using 18F salts within a short reaction time, and with temperature and solvent conditions that are mild for the tracer molecule. The method provides a quick and convenient process that maintains the biological activities of the target molecules. The radio-labeled biomolecules may be used as contrast agents for Positron Emission Tomography (PET).
Claims15
A method of preparing an 18F-labeled radiotracer for use in positron emission tomography (PET), said method comprising:
- a. providing a target tracer compound having a biological moiety;
- b. providing a non-radioactive, water-soluble perfluoroaryl (PFA) compound;
- c. reacting the target tracer compound and the water-soluble PFA compound in a first aqueous solvent that is predominantly water, at a first ambient temperature that is mild for the biological moiety, thereby forming a PFA-modified target tracer compound, wherein the PFA-modified target tracer compound is non-radioactive, and wherein a biological activity of the biological moiety is preserved;
- d. providing an 18F salt;
- and
- e. reacting the 18F salt with the PFA-modified target tracer compound in a second aqueous solvent that is predominantly water, at a second ambient temperature that is mild for the biological moiety, wherein an 18F radioisotope of the 18F salt replaces an 19F isotope of the PFA-modified target tracer compound, thereby forming the 18F-labeled
- wherein the first ambient temperature and the second ambient temperature is at most about 37° C., wherein the biological activity of the biological moiety is preserved.
Dependencies are inferred from the claim text.