99mTc-labeled isonitrile-containing glucose derivative and preparation method and use thereof

A method of preparing a 99mTc-labeled isonitrile-containing glucose derivative (“CNDG”) having a structural formula (I) of [99mTc-(CNDG)6]+, wherein formula (I) is as follows:

• wherein, in the structural formula (I),
• a 99mTc+ core is a central nucleus,
• the carbon atom of isonitrile in the CNDG coordinates with 99mTc(I) to form a hexacoordinated complex [99mTc-(CNDG)6]+, and
• n is an integer greater than two (2),
• the method comprising:
• (a) synthesizing a CNDG ligand by a method comprising:
• weighing an appropriate amount of glucosamine hydrochloride;
• adding anhydrous methanol to dissolve glucosamine hydrochloride to produce a methanol solution;
• adding an appropriate amount of NaOH;
• allowing a reaction between NaOH and glucosamine hydrochloride to proceed at room temperature for about 30 minutes;
• placing the reaction in an ice water bath;
• adding a methanol solution comprising a compound of structural formula (II) dropwise to the reaction in the ice water bath for three hours after addition is completed to produce the CNDG ligand, the compound of the structural formula (II) as follows:
• wherein n is an integer greater than two (2);
• and
• removing the solvent from the CNDG ligand upon completion of the reaction;
• (b) producing a solution of CNDG ligand, SnCl2.2H2O and sodium citrate dissolved in redistilled water, wherein a pH of the solution is adjusted to a pH from 5 to 6 after dissolution, wherein an appropriate volume of the solution is dispensed into a receptacle;
• (c) lyophilizing the solution in the receptacle to produce a lyophilized CNDG kit;
• (d) adding freshly washed Na99mTcO4 to the lyophilized CNDG kit;
• and
• (e) heating the solution containing the CNDG kit contents and Na99mTcO4 to produce the [99mTc-(CNDG)6]+.