Antibody-drug conjugates comprising substituted benzo[e]pyrrolo[1,2-α][1,4]diazepines

An antibody-drug conjugate represented by [Formula 25], [Formula 26], [Formula 27], or [Formula 28], wherein:

• [Formula 28] is:
• wherein:
• m2 represents 1 or 2;
• Ab represents an immunoglobulin G (IgG) antibody or a functional fragment of the immunoglobulin G (IgG) antibody;
• and
• the N297 glycan of Ab represents:
• (i) N297-(Fuc)MSG1 of [Formula 29], N297-(Fuc)MSG2 of [Formula 30], or N297-(Fuc)SG of [Formula 31]:
• wherein:
• L(PEG) represents —NH—CH2CH2—(OCH2CH2)3—*, wherein the —NH— at the left end is bound via an amide bond to the carboxylic acid at the 2-position of a sialic acid at the non-reducing terminal in one or both of the 1-3 and 1-6 branched chains of the β-Man in the N297 glycan, and further wherein each * represents bonding to a nitrogen atom at the 1-position or 3-position of the triazolyl in [Formula 25], [Formula 26], [Formula 27], or [Formula 28];
• and
• each represents bonding to Asn297 of the antibody;
• or
• (ii) a mixture of N297-(Fuc)MSG1 of [Formula 29] and N297-(Fuc)MSG2 of [Formula 30] above.