Method of extracting active ingredients in mushrooms

An extraction method for mushroom, comprising steps of:

• a crushing step for crushing a mushroom by a crushing machine to obtain a crude mushroom crush;
• a mixing step for mixing the crude mushroom crush with deionized water to obtain a crude crush mixture, wherein the crude mushroom crush and the deionized water are fully mixed in a mass-volume ratio of 1:400 to 10:400;
• a subcritical fluid extraction step for adding the crude crush mixture into a subcritical fluid extractor and extracting the crude crush mixture to obtain a crude mushroom extract, wherein a pressure in the subcritical fluid extractor is between 1000 psi and 5000 psi, an extraction temperature is between 50° C. and 200° C., and an extraction time is between 5 minutes and 40 minutes;
• a first centrifugal step for centrifuging the crude mushroom extract under 5000˜15000 rpm for 5˜30 minutes, collecting a supernatant separated from the crude mushroom extract to obtain a first supernatant, and collecting a precipitate separated from the crude mushroom extract to obtain a mushroom residue;
• a mushroom residue mixing step for fully mixing the mushroom residue with deionized water and a cellulase enzyme to obtain a reaction mixture;
• a high-pressure hydrolysis step for adding the reaction mixture into a high-pressure hydrolysis reactor and extracting the reaction mixture to obtain a hydrolysate, wherein a pressure in the high-pressure hydrolysis reactor is between 500 psi and 5000 psi, an extraction temperature is between 10° C. and 100° C., and an extraction time is between 10 minutes and 100 minutes;
• a second centrifugal step for centrifuging the hydrolysate under 5000˜15000 rpm for 5˜30 minutes, and collecting a supernatant separated from the hydrolysate to obtain a second supernatant;
• and
• a concentration step for combining the first supernatant and the second supernatant, and then concentrating a mixture of the first supernatant and the second supernatant under 37° C. to obtain a mushroom extraction solution;
• wherein the mushroom is Agaricus blazei Murill, Phellinus linteus, or Tremella fuciformis.