Compositions and methods for enzyme immobilization
AssigneeAgroSpheres, Inc.
InventorsAmeer Hamza Shakeel, Zachery George Davis, Joseph Thomas Frank, Sepehr Zomorodi, Payam Pourtaheri
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Abstract
The present disclosure relates to compositions of immobilized enzymes on the surface of achromosomal and/or anucleate cells and uses thereof. In particular, the present disclosure provides genetically engineered minicells with enzymes self-assembled on their surface. The immobilized enzymes on the surface of achromosomal and/or anucleate minicells, has agricultural, industrial, and environmental applications due to their improved stability durability and, reusability. Also, provided are methods for producing and purifying enzyme-immobilized minicells.
Claims36
An industrially suitable anucleated cell-based enzyme immobilization and delivery platform, comprising:
- an intact anucleated cell derived from a protease deficient parental cell, wherein said anucleated cell comprises
- (i) at least one expressed self-assembled enzyme immobilized to the surface of said cell and
- (ii) at least one fusion protein comprising at least one surface expressing moiety and at least one cell adhesion moiety.
An industrially suitable anucleated cell-based enzyme immobilization and delivery platform, comprising:
- an intact anucleated cell derived from a protease deficient parental cell, wherein the anucleated cell comprises at least two different expressed self-assembled enzymes immobilized to a surface of said cell, wherein each of the expressed self-assembled enzymes is a fusion protein comprising at least one surface expressing moiety and at least one enzymatically active moiety, and wherein said enzymatically active moiety of a first expressed self-assembled enzyme is lipase and said enzymatically active moiety of a second expressed self-assembled enzyme is not lipase.
The anucleated cell-based enzyme immobilization and delivery platform according to 11, wherein the at least two different expressed self-assembled enzymes have a complimentary function.
The anucleated cell-based enzyme immobilization and delivery platform according to 11, wherein the at least two different expressed self-assembled enzymes act synergistically.
The anucleated cell-based enzyme immobilization and delivery platform according to 11, wherein the at least two different expressed self-assembled enzymes each work to carry out a portion of an overall enzymatic reaction.
An industrially suitable anucleated cell-based enzyme immobilization and delivery platform, comprising:
- an intact anucleated cell derived from a protease deficient parental cell, wherein the anucleated cell comprises at least two different expressed self-assembled enzymes immobilized to a surface of said cell, wherein each of the expressed self-assembled enzymes is a fusion protein comprising at least one surface expressing moiety and at least one enzymatically active moiety, and wherein said enzymatically active moiety of a first expressed self-assembled enzyme is glucose isomerase and said enzymatically active moiety of a second expressed self-assembled enzyme is not glucose isomerase.
The anucleated cell-based enzyme immobilization and delivery platform according to 20, wherein the at least two different expressed self-assembled enzymes have a complimentary function.
The anucleated cell-based enzyme immobilization and delivery platform according to 20, wherein the at least two different expressed self-assembled enzymes act synergistically.
The anucleated cell-based enzyme immobilization and delivery platform according to 20, wherein the at least two different expressed self-assembled enzymes each work to carry out a portion of an overall enzymatic reaction.
An industrially suitable anucleated cell-based enzyme immobilization and delivery platform, comprising:
- an intact anucleated cell derived from a protease deficient parental cell, wherein the anucleated cell comprises at least two different expressed self-assembled enzymes immobilized to a surface of said cell, wherein each of the expressed self-assembled enzymes is a fusion protein comprising at least one surface expressing moiety and at least one enzymatically active moiety, and wherein said enzymatically active moiety of a first expressed self-assembled enzyme is protease and said enzymatically active moiety of a second expressed self-assembled enzyme is not protease.
The anucleated cell-based enzyme immobilization and delivery platform according to 28, wherein the at least two different expressed self-assembled enzymes have a complimentary function.
The anucleated cell-based enzyme immobilization and delivery platform according to 28, wherein the at least two different expressed self-assembled enzymes act synergistically.
The anucleated cell-based enzyme immobilization and delivery platform according to 28, wherein the at least two different expressed self-assembled enzymes each work to carry out a portion of an overall enzymatic reaction.
Dependencies are inferred from the claim text.