Glycerol free ethanol production

A recombinant cell, optionally a recombinant yeast cell comprising:

• a) one or more heterologous genes encoding a glycerol dehydrogenase;
• b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29);
• c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO);
• and
• d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK);
• e) optionally, one or more heterologous genes encoding a glycerol transporter;
• and
• f) a deletion or disruption of one or more endogenous nucleotide sequences encoding a glycerol exporter, wherein the deleted or disrupted endogenous nucleotide sequence encoding a glycerol exporter is a nucleotide sequence encoding FPS1.