Method for producing cartilage cells induced to be differentiated from stem cells

A method for producing chondrocytes obtained by differentiation induction from stem cells, comprising:

• i) culturing induced pluripotent stem cells (iPSCs) to obtain embryoid bodies, wherein the induced pluripotent stem cells of step i) are obtained by reprogramming cord blood mononuclear cells;
• ii) performing adherent culture of the embryoid bodies obtained in step i), to obtain outgrowth cells (OG cells);
• iii) performing centrifugation of the OG cells obtained in step ii) so that the OG cells are isolated by sizes, and selecting light OG cells;
• iv) inducing differentiation of the light OG cells selected in step iii) into chondrocytes;
• and
• v) obtaining the chondrocytes produced by differentiation induction in step iv),
• wherein the centrifugation and selection in step iii) is performed through the following steps a) to c):
• a) centrifuging a medium containing the OG cells at 300 rpm to 800 rpm for 3 to 10 seconds, to classify precipitated cells as heavy OG cells;
• b) centrifuging the supernatant after the centrifugation in step a) at 800 rpm to 1,200 rpm for 3 to 10 seconds, to classify precipitated cells as medium OG cells;
• and
• c) centrifuging the supernatant after the centrifugation in step b) at 1,200 rpm to 2,000 rpm for 3 to 10 seconds, to classify precipitated cells as the light OG cells, and
• wherein the light OG cells exhibit higher expression level of SOX9 and lower expression level of COL10 than the heavy OG cells and the medium OG cells.