Method for preparation of xanthan gum copolymer nanomicelles

A method of making anti-cancer drug-loaded nanoparticles comprising:

• a) obtaining xanthan gum copolymer nanomicelles;
• b) freeze-drying the xanthan gum copolymer nanomicelles to obtain copolymer nanomicelles powder;
• c) dissolving an anticancer compound in methanol to make an anticancer drug solution;
• d) mixing the copolymer nanomicelles powder of step b) with the anticancer drug solution of step c) to obtain a mixture;
• and
• e) dialyzing the mixture of step d) to obtain the anti-cancer drug-loaded nanoparticles,
• wherein the step a) comprises:
• 1) degrading xanthan gum in an aqueous solution so that dynamic viscosity of the xanthan gum aqueous solution is reduced as compared with non-degraded xanthan gum, then conducting drying, crushing and sieving to obtain degraded xanthan gum;
• 2) dissolving the degraded xanthan gum obtained in step 1) in N, N-dimethylformamide to obtain a degraded xanthan gum solution, dissolving 2-bromoisobutyryl bromide in N, N-dimethylformamide in an ice bath under stirring to prepare 2-bromoisobutyryl bromide solution, mixing the 2-bromoisobutyryl bromide solution with the degraded xanthan gum solution and then triethylamine to carry out a reaction at room temperature, then conducting precipitation with ether, filtering, and drying to obtain xanthan gum bromide;
• 3) dissolving the xanthan gum bromide obtained in step 2) in N, N-dimethylformamide to obtain a xanthan gum bromide solution in N, N-dimethylformamide, mixing diacetone acrylamide with the xanthan gum bromide solution in N, N-dimethylformamide in a reaction vessel, replacing air in the reaction vessel with nitrogen, then adding tri [2-(dimethylamino) ethyl] amine and cuprous chloride to the reaction vessel to carry out reaction at 55-65° C. for 3-5 hours, conducting precipitation by ether, filtering, washing, and drying to obtain xanthan gum copolymer, and
• 4) dissolving the xanthan gum copolymer of step 3) in a polar organic solvent, then conducting filtering with a microporous filtration membrane to obtain filtered xanthan gum copolymer solution, adding water to the filtered xanthan gum copolymer solution until a micellar solution is formed, dialyzing the micellar solution in deionized water to obtain the gum copolymer nanomicelles.