Method for producing a nanostructured complex (CFI-1), a protein-associated nanostructured complex (MRB-CFI-1) and use

A process of obtaining a nanostructured complex (CFI-1), by chemical synthesis, comprising the following steps:

• (a) Preparing ammonium phosphate dibasic [(NH4)2HPO4] in situ in the presence of ammonia and orthophosphoric acid, in a mechanical homogenizer with a minimum power of 500 W, at a temperature range between 25 and 55° C. up to neutralization;
• (b) Mixing two salts:
• magnesium chloride, at 1-3% (by mass) and the ammonium phosphate dibasic obtained in step (a) at concentration of 1-4% (by mass) and at a temperature between 22 and 30° C. and pH 5-7 in a mechanical homogenizer with a minimum power of 500 W in a variable rotation range between 7000 and 15000 rpm;
• (c) Applying a pressure level to the mixture obtained in step (b) in a high pressure homogenizer with a homogenization valve with variable pressure between 400 and 700 bar, and in the second stage, the homogenizing valve with variable pressure between 50 and 70 bar for up to 1 to 3 cycles;
• (d) Cooling the suspension obtained at step (c) to a temperature of between 0 and 20° C.;
• (e) Precipitating crystals from the suspension obtained in step (d);
• and
• (f) Washing the crystals obtained in step (e) with distilled and sterile water and drying at a temperature between 30 and 40° C. for a time interval between 24 and 72 hours.