Time-resolved fluorescence immunochromatographic test paper card for testing clothianidin

An immunochromatographic test paper card for testing clothianidin, comprising:

• a base plate;
• a sample pad, a binding pad, a nitrocellulose (NC) film, and an absorption pad that are sequentially arranged on the base plate, wherein:
• the binding pad comprises a detection microsphere and a quality control microsphere thereon, wherein the detection microsphere is a fluorescent microsphere coated with a clothianidin monoclonal antibody, and the quality control microsphere is a fluorescent microsphere coated with a rabbit anti-tag protein;
• the NC film is provided with a detection line and a control line thereon, the lengths of the detection line and the control line are the same as the width of the NC film, the detection line is coated with a clothianidin antigen, and the quality control line is coated with an anti-rabbit antibody;
• a first clamping part is arranged at one end of the sample pad;
• a second clamping part and a third clamping part are respectively arranged at two ends of the binding pad;
• a fourth clamping part is arranged at one end of the NC film, and the other end of the NC film is joined with the absorption pad;
• and the first clamping part, the second clamping part, the third clamping part and the fourth clamping part are all of semi-arc structures;
• the first clamping part and the second clamping part fit to each other, and the third clamping part and the fourth clamping part fit to each other;
• wherein, the first clamping part and the sample pad;
• the second clamping part, the third clamping part and the binding pad;
• and the fourth clamping part and the NC film are all integrally formed structures.