Method for the production of xylobiose and other defined xylooligosaccharides
OwnerFranz Josef St. John, Merritt Evan Casey Crooksinventors
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Abstract
The present invention provides compositions, methods, and kits based on a novel two-enzyme system. This system uses a combination of an appendage dependent endoxylanase and xylobiohydrolase activity to produce xylobiose and xylan-derived oligosaccharides using lignocellulosic biomass material, an enriched xylan fraction thereof, or an extracted, purified xylan material as a starting material.
Claims19
A composition configured to produce xylobiose, 22-(4-O-methyl-α-D-glucuronosyl)-xylobiose [aldotriuronic acid], and 22-(4-O-methyl-α-D-glucuronosyl)-xylotriose [aldotetrauronic acid], the composition comprising:
- a) an isolated appendage dependent endoxylanase that is a canonical glycoside hydrolase family 30 subfamily 8 (GH30-8) enzyme having at least 90% identity to a sequence selected from the group consisting of SEQ ID NO:
- 14, 18, and 19;
- and
- b) an isolated xylobiohydrolase comprising an amino acid sequence with at least 90% identity to a sequence selected from the group consisting of SEQ ID NO:
- 1, 9, and 10 or a portion thereof with xylobiohydrolase activity.
A method for producing xylobiose, the method comprising contacting a lignocellulosic biomass material, an enriched xylan fraction thereof, or an extracted, purified xylan material with:
- a) an isolated appendage dependent endoxylanase that is a canonical glycoside hydrolase family 30 subfamily 8 (GH30-8) enzyme having at least 90% identity to a sequence selected from the group consisting of SEQ ID NO:
- 14, 18, and 19;
- and
- b) an isolated xylobiohydrolase comprising an amino acid sequence with at least 90% identity to a sequence selected from the group consisting of SEQ ID NO:
- 1, 9, and 10 or a portion thereof with xylobiohydrolase activity;
- thereby producing a product mixture comprising xylobiose, 22-(4-O-methyl-α-D-glucuronosyl)-xylobiose [aldotriuronic acid], and 22-(4-O-methyl-α-D-glucuronosyl)-xylotriose [aldotetrauronic acid].
Dependencies are inferred from the claim text.