US 11,618,913 B2 · Method for preparing xyloglucan-oligosaccharides

Abstract

The present invention relates to a method for preparing oligosaccharides which can be used among others as food additives to reduce calorie content, to sweeten food products, to increase the fiber content of food products, to improve the texture of food products and to stimulate the gut microbiome bacteria. Furthermore they can be applied in the fields of animal feed, or other applications. More particularly, this invention is directed to a high temperature hydrolysis of xyloglucan polysaccharide to defined xyloglucan oligosaccharides. The invention further relates to oligosaccharide hydrolysates produced with the method of the invention and to the use of said oligosaccharide hydrolysates in human and/or animal nutrition, as prebiotic or other uses. Further provided are novel endoglucanases for use in the method of the invention as well as in other applications.

Claims8

Dependent claims

A method for producing xyloglucan oligosaccharides (XGOS) from a xyloglucan source, said method comprising:

contacting, at a temperature in the range of 50.5° C. to 80° C., a xyloglucan polysaccharide with an enzyme that is a polypeptide selected from the group consisting of SEQ ID NO:
2, SEQ ID NO:
3, and SEQ ID NO:
4,
wherein the enzyme exhibits xyloglucanase activity at a temperature higher than 50° C., and exhibits an end product inhibition constant (Ki) of at least 5 mM.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/765,906Filed 16 Nov 2018

Priority

EP 1720308722 Nov 2017

Published

US 2020/0362379 A119 Nov 2020

Granted

4 Apr 2023

Term ends

Not estimatedThe source records don’t support an estimate.

Classification13 codes

C12P 19/14
Preparation of compounds containing saccharide radicals: produced by the action of a carbohydrase , e.g. by alpha-amylase , e.g. by cellulase,…
C12Y 302/01004
Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds: cellulase , i.e. endo-1,4-beta-glucanase
A23L 29/206
Containing gelling or thickening agents: of vegetable origin
A61K 31/702
Carbohydrates; Sugars; Derivatives thereof: oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic…

Method for preparing xyloglucan-oligosaccharides

Life of this patent

Method for preparing xyloglucan-oligosaccharides

Life of this patent

Abstract

Claims8