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US 11,618,906 B2Granted
Hydroxylation techniques
AssigneeHypha Discovery Limited
InventorsJonathan Charles Paul Steele, Antonio De Riso, Francesco Falcioni, Richard Kerry Phipps, Stephen Keith Wrigley, Kinga Linda Nytko, Vincent Poon, Sebastian Schulz, John Maxim Ward, Mariacristina Bawn
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Abstract
The use of a cytochrome P-450 enzyme comprising SEQ ID NO: 110, or a variant enzyme having at least 70% identity thereto and having CYP-450 activity, for the hydroxylation of an organic compound, wherein the amino acid residue at position 291 is not threonine.
Claims20
A kit comprising:
- i) an enzyme having cytochrome P-450 activity and comprising the amino acid sequence set forth in SEQ ID NO:
- 110, or a sequence having at least 90% identity thereto, wherein the amino acid residue at the position corresponding to position 291 of SEQ ID NO:
- 110 is aspartic acid, glutamic acid, asparagine, glutamine, valine, methionine, phenylalanine, tryptophan, isoleucine, proline or histidine;
- or
- ii) a microorganism that expresses an enzyme as defined in (i);
- or
- iii) an extract of the microorganism of (ii).
4 more elements
An enzyme having cytochrome P-450 activity and comprising the amino acid sequence set forth in SEQ ID NO:
- 110, or a sequence with at least 90% sequence identity thereto, wherein the amino acid residue at the position corresponding to position 291 of SEQ ID NO:
- 110 is aspartic acid, glutamic acid, asparagine, glutamine, valine, methionine, phenylalanine, tryptophan, isoleucine, proline or histidine.
Dependencies are inferred from the claim text.