Beta-ketoacyl-ACP synthase II variants
AssigneeCorbion Biotech, Inc.
InventorsNien-Hsi Ko, Joshua Ferreira, Janice Lau Wee, William E. Maffe, Douglas A. Hattendorf
Life of this patent
Estimating the term…
Abstract
Provided are non-natural or variant beta-ketoacyl-acyl carrier protein (ACP) synthase (KAS) II enzymes (KASII), polynucleotides encoding such variant KASII, host cells expressing such variant KASII, oils and oil products produced by such cells, and methods of making and using such variant KASII.
Claims22
A polynucleotide encoding a non-natural variant β-ketoacyl-acyl carrier protein (ACP) synthase (KAS) II enzyme (KASII), wherein the non-natural KASII comprises at least about 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to amino acid residues 39-469 of SEQ ID NO:2 and comprises an X at the position corresponding to position 162;
- wherein X is an amino acid residue selected from the group consisting of cysteine (C), aspartate (D), glutamate (E), phenylalanine (F), glycine (G), histidine (H), isoleucine (I), lysine (K), leucine (L), methionine (M), asparagine (N), proline (P), glutamine (Q), arginine (R), serine (S), threonine (T), valine (V), tryptophan (W) and tyrosine (Y), wherein the positions are with reference to SEQ ID NO:2, and wherein the non-natural KASII catalyzes the elongation of C14 acyl-ACP to C16 acyl-ACP or C16 acyl-ACP to C18 acyl-ACP.
Dependencies are inferred from the claim text.