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US 11,618,769 B2Granted
Antibody purification
OwnerRégine Freichels, Olivier Favre-Bulle +2inventors
InventorsRégine Freichels, Olivier Favre-Bulle, François Côte, Bertrand Mérot
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Abstract
The present invention relates to methods for purifying heterologous antibodies from caprine milk, such as goat milk. The present invention includes protein A based affinity chromatography to obtain purified transgenically expressed antibodies, in particular concentrates free from endogenous caprine antibodies.
Claims14
A method for purifying antibodies, the method comprising:
- providing caprine whey comprising a monoclonal heterologous antibody;
- contacting the caprine whey with a protein A containing matrix;
- separating the protein A containing solid matrix from the caprine whey;
- and
- eluting the heterologous antibody from the protein A containing matrix;
- wherein, prior to eluting the heterologous antibody, endogenous caprine antibodies are eluted at a pH which is higher than the pH for eluting the heterologous antibodies;
- and
- wherein
- endogenous caprine antibodies are eluted at a pH ranging from 4.0 to 5.0;
- and/or
- heterologous antibodies are eluted at a pH ranging from 2.0 to 4.0;
- and/or
- the protein A containing matrix and/or the caprine whey is equilibrated to a pH ranging from 6 to 7 before contacting the caprine whey with the protein A containing matrix.
10 more elements
Dependencies are inferred from the claim text.