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US 11,613,771 B2Granted
Methods for delivering an analyte to transmembrane pores
AssigneeOxford Nanopore Technologies PLC
InventorsClive Gavin Brown, Daniel Ryan Garalde, Andrew John Heron, Daniel John Turner, James White
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Abstract
The invention relates to a new method of delivering an analyte to a transmembrane pore in a membrane. The method involves the use of microparticles.
Claims14
A method for delivering a polynucleotide to a transmembrane pore in a membrane and characterising the polynucleotide, the method comprising:
- (a) providing the polynucleotide attached to a microparticle;
- (b) delivering the microparticle towards the membrane and thereby delivering the polynucleotide to the transmembrane pore, wherein the method comprises the microparticle moving (i) along the membrane;
- and/or (ii) in parallel with the membrane;
- (c) allowing the polynucleotide to interact with the transmembrane pore such that the polynucleotide moves through the pore, and using a polynucleotide binding protein to control the movement of the polynucleotide through the pore;
- and
- (d) taking one or more measurements as the polynucleotide moves with respect to the pore, wherein the measurements are indicative of one or more characteristics of the polynucleotide, and thereby characterising the polynucleotide;
- wherein the polynucleotide detaches from the microparticle when interacting with the pore.
4 more elements
Dependencies are inferred from the claim text.