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US 11,613,768 B2Granted
Microbial production of 2-phenylethanol from renewable substrates
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsDavid Nielsen, Michael Machas, Rebekah McKenna
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Abstract
Described herein are engineered metabolic pathways in recombinant microorganism host cells which result in the production of 2-phenylethanol or 2-phenylacetic acid. Also described herein are methods of using the recombinant microorganisms for the production of 2-phenylethanol or 2-phenylacetic acid.
Claims14
A recombinant organism comprising,
- (i) at least one heterologous gene encoding an enzyme having phenylalanine ammonia lyase (PAL) activity,
- (ii) at least one heterologous gene encoding an enzyme having trans-cinnamic acid decarboxylase (CADC) activity,
- (iii) at least one heterologous gene encoding an enzyme having styrene monooxygenase (SMO) activity,
- (iv) at least one heterologous gene encoding an enzyme having styrene oxide isomerase (SOI) activity, and
- (v) at least one gene encoding an enzyme having 2-phenylacetaldehyde reductase (PAR) activity,
- wherein the organism is capable of producing 2-phenylethanol from a fermentable carbon substrate,
- wherein the organism is not capable of producing 2-phenylacetic acid by an enzyme having 2-phenylacetaldehyde dehydrogenase (PADH) activity.
4 more elements
Dependencies are inferred from the claim text.