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AssigneeWuhan Kangfude Biotechnology Co., Ltd.
InventorsWei Wang, Xiaofeng Wang, Yanhong Liu, He Huang, Huoqing Chen, Xianqiao Chen
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The present invention relates to a recombinant OxDC expressed in a filamentous fungal host cell, methods for constructing a recombinant filamentous fungal host cell, methods for producing recombinant OxDC and the application thereof. The recombinant filamentous fungal host cell comprises one or more copies of OxDC expression cassette integrated in its genome; the expression cassette comprises a promoter, a signal peptide coding sequence, an OxDC coding sequence and a transcription terminator. The host cell can be constructed by random integration or site-specific integration. In addition, the present invention also optimizes the medium formulation for different recombinant filamentous fungal host cells. In the production of the recombinant OxDC, the final yield and enzyme activity were greatly improved. The invention effectively solves the problem that the production of OxDC in the prior art cannot be industrialized on a large scale.
A recombinant oxalate decarboxylase, wherein the recombinant oxalate decarboxylase is recombinantly expressed in a filamentous fungal host cell, resulting in a form and degree of glycosylation different from an original oxalate decarboxylase expressed in an original host cell, wherein the form and degree of glycosylation of the recombinant oxalate decarboxylase is specific to the filamentous fungal host cell, wherein the recombinant oxalate decarboxylase consists of the amino acids 20 to 470 of SEQ ID NO:
Dependencies are inferred from the claim text.