Isolation of bona fide pancreatic progenitor cells
AssigneeUniversity of Copenhagen
InventorsJacqueline Ameri, Henrik Semb
Life of this patent
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Abstract
The present invention relates to a method for isolating bona fide pancreatic progenitor cells and to cell populations enriched for bona fide pancreatic progenitor cells.
Claims18
A method for isolating a cell population enriched for bona fide pancreatic progenitor cells, the method comprising:
- i) providing a cell population comprising at least one bona fide pancreatic progenitor cell, wherein the bona fide pancreatic progenitor cell expresses PDX1 and NKX6-1;
- and
- ii) exposing the cell population to a ligand which binds to a marker specific for PDX1+NKX6-1+ cells, wherein the marker is selected from SCN9A, NAALADL2, KCNIP1 and CALB1, and selecting the cells that bind to the ligand from the cells that do not bind to the ligand, thereby enriching the cell population for PDX1+NKX6-1+ cells;
- thereby obtaining a cell population enriched for bona fide pancreatic progenitor cells.
A cell population comprising at least 50% bona fide pancreatic progenitor cells, such as at least 75% bona fide pancreatic progenitor cells, such as at least 80% bona fide pancreatic progenitor cells, such as at least 85% bona fide pancreatic progenitor cells, such as at least 90% bona fide pancreatic progenitor cells, wherein at least 34% of the cells of the cell population express GP2.
Dependencies are inferred from the claim text.