Method for purifying mesenchymal stem cells to improve transplantation efficiency
AssigneesMedical & Biological Laboratories Co., Ltd., Jsr Corporation
InventorsChihiro Akazawa, Eriko Grace Suto, Yo Mabuchi
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Abstract
A method for purifying and enriching mesenchymal stem cells (MSCs) simply and efficiently. Separation of cells expressing CD73 protein on the surface from fresh tissue isolated from a living body allows purification and enrichment of MSCs easily and efficiently. MSCs may be selectively isolated with a single antibody before culturing to establish a culture system of mesenchymal stem cells that enhances the engraftment efficiency in the transplanted site.
Claims11
A method for purifying mesenchymal stem cells (MSCs), comprising:
- i) preparing a population of fresh cells isolated from a living organism,
- ii) isolating cells that express the CD73 protein on their surfaces and do not express a single combination of markers selected from the group consisting of CD31/CD45/GPA, CD31/CD45/Ter119, and CD31/CD45 to obtain isolated cells, wherein no other negative or positive selection markers are used to select the cells for isolation.
A method for purifying mesenchymal stem cells (MSCs), comprising:
- i) preparing a population of fresh cells isolated from a living organism;
- ii) isolating cells that express the CD73 protein on their surfaces, and using CD73+ as the sole selection marker.
Dependencies are inferred from the claim text.