Perfusion bioreactor platform

A cell culture apparatus comprising a plurality of plates stacked on one another, each of said plurality of plates having a first major surface and an opposing second major surface, wherein the first major surface comprises a plurality of microwells, wherein the plurality of microwells has a cross-sectional shape approximating a sine wave;

• and
• a plurality of spacers extending from the first major surface, wherein a plurality of cell culture media flow channels are defined between adjacent plates, wherein the plurality of plates are stacked to align spacers of adjacent plates, the aligned spacers defining walls extending from a bottom-most plate to a top-most plate, wherein each of the spacers comprises a plurality of first air channels disposed along the length of the spacer, wherein at least one wall of the walls defines a plurality of second air channels extending from the bottom-most plate to the top-most plate, wherein the second air channels are formed by the aligned spacers aligning the first air channels of the adjacent spacers, wherein the first and second air channels are in gaseous communication with an exterior of the cell culture apparatus and are in gaseous communication with at least one of the plurality of cell culture media flow channels.