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US 11,613,561 B2Granted
Artificial alphavirus-derived RNA replicon expression systems
AssigneeTIBA Biotech LLC
InventorsJasdave S Chahal, Justine S McPartlan
Life of this patent
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Abstract
Synthetic alphavirus-derived replicon expression systems comprising nucleic acid sequences encoding at least one modified nonstructural protein, and synthetic nucleic acid sequences encoding at least one heterologous protein are described. Methods of producing at least one heterologous protein in a cell, or of inducing an immune response in a subject by administering and/or expressing the synthetic alphavirus-derived replicon expression systems are provided.
Claims17
A synthetic alphavirus-derived replicon nucleic acid molecule comprising:
- (i) a first nucleic acid encoding alphavirus nonstructural proteins nsP1, nsP2, nsP3, and nsP4, and comprising at least one silent mutation introduced within a region from nt 6381 to nt 7083, in the sequence of the alphavirus genome as set forth in SEQ ID NO:
- 17 and comprising a polynucleotide sequence with at least 90% identity to the reference sequence as set forth in SEQ ID NO:
- 18;
- and (ii) a second nucleic acid comprising a modified subgenomic open reading frame (ORF).
1 more element
Dependencies are inferred from the claim text.