Kits to detect adenovirus nucleic acids

A kit containing a detection probe configured to hybridize to a portion of an Adenovirus nucleic acid or to a portion of an amplicon generated therefrom, wherein the detection probe comprises a target hybridizing sequence selected from the group consisting of:

• SEQ ID NO:10, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:29, and SEQ ID NO:36,
• wherein the detection probe further comprises one or more of
• (a) a label,
• (b) a substitution at the 2′ position of at least one ribose moiety, and
• (c) a blocking moiety at or near the 3′ end of the detection probe, wherein the blocking moiety is configured to prevent enzyme-mediated extension of the detection probe in an amplification reaction, and
• wherein the kit further comprises at least one amplification oligonucleotide, wherein:
• (i) if the detection probe comprises the target hybridizing sequence of SEQ ID NO:10, then the at least one amplification oligonucleotide is a forward primer comprising the target hybridizing sequence of SEQ ID NO:5 and a reverse primer comprising the target hybridizing sequence of SEQ ID NO:6 or SEQ ID NO:8;
• (ii) if the detection probe comprises the target hybridizing sequence of SEQ ID NO:17, then the at least one amplification oligonucleotide is a forward primer comprising the target hybridizing sequence of SEQ ID NO:11 or SEQ ID NO:12 and a reverse primer comprising the target hybridizing sequence of SEQ ID NO:13 or SEQ ID NO:15;
• (iii) if the detection probe comprises the target hybridizing sequence of SEQ ID NO:19, then the at least one amplification oligonucleotide is a forward primer comprising the target hybridizing sequence of SEQ ID NO:11 or SEQ ID NO:12 and a reverse primer comprising the target hybridizing sequence of SEQ ID NO:13 or SEQ ID NO:15;
• (iv) if the detection probe comprises the target hybridizing sequence of SEQ ID NO:21, then the at least one amplification oligonucleotide is two forward primers comprising the target hybridizing sequences of SEQ ID NO:25 and SEQ ID NO:26 and two reverse primers comprising the target hybridizing sequences of SEQ ID NO:27 and SEQ ID NO:28;
• (v) if the detection probe comprises the target hybridizing sequence of SEQ ID NO:23, then the at least one amplification oligonucleotide is two forward primers comprising the target hybridizing sequences of SEQ ID NO:25 and SEQ ID NO:26 and two reverse primers comprising the target hybridizing sequences of SEQ ID NO:27 and SEQ ID NO:28;
• or
• (vi) if the detection probe comprises the target hybridizing sequence of SEQ ID NO:29, then the at least one amplification oligonucleotide is two forward primers comprising the target hybridizing sequences of SEQ ID NO:25 and SEQ ID NO:26 and two reverse primers comprising the target hybridizing sequences of SEQ ID NO:27 and SEQ ID NO:28.

A kit containing at least two detection probes configured to hybridize to a portion of an Adenovirus nucleic acid or to a portion of an amplicon generated therefrom, wherein each detection probe comprises a target hybridizing sequence selected from the group consisting of SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:36, and SEQ ID NO:37,

• wherein each of the detection probes further comprises one or more of
• (a) a label,
• (b) a substitution at the 2′ position of at least one ribose moiety, and
• (c) a blocking moiety at or near the 3′ end of the detection probe, wherein the blocking moiety is configured to prevent enzyme-mediated extension of the detection probe in an amplification reaction, and
• wherein at least one of the at least two detection probes comprises the target hybridizing sequence of SEQ ID NO:21 or SEQ ID NO:36.