Back to results
US 11,608,504 B2Granted
Nucleic acid construct for in vitro and in vivo gene expression
AssigneeGlycom A/S
InventorsMargit Pedersen, Manos Papadakis
Life of this patent
Estimating the term…
Abstract
The present invention relates to the field of recombining production of biological molecules in host cells. The invention provides nucleic acid constructs that allow to modify expression of a desired gene using both in vitro and in vivo gene expression systems. The constructs can advantageously be used to produce a variety of biological molecules recombinantly in industrial scales, e.g. human milk oligosaccharides (HMO).
Claims20
A synthetic nucleic acid construct comprising a synthetic non-coding DNA sequence (i) that comprises a first DNA fragment and a second DNA fragment,
- wherein the first DNA fragment is a DNA sequence derived from the 5′-untranslated region (5′-UTR) of a glp gene of Escherichia coli and is located upstream from the second DNA fragment;
- and
- wherein the nucleotide sequence of the second DNA fragment is selected from the group consisting of SEQ ID NO:
- 10, 38, 39, 40, 41, 42, 43, 44, 45, 46 and 47.
1 more element
Dependencies are inferred from the claim text.